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High-Throughput Screening Assays in Toxicology

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Part I: In Vitro Toxicological High-Thoughput Screening Methods 1. Monitoring Ligand-Activated Protein-Protein Interactions Using Bioluminescent Resonance Energy Transfer (BRET) Assay Carlos Coriano, Emily Powell, and Wei Xu 2. Mitochondrial Membrane Potential Assay Srilatha Sakamuru, Matias S. Attene-Ramos, and Menghang Xia 3. Using beta-Lactamase and NanoLuc Luciferase Reporter Gene Assays to Identify Inhibitors of the HIF-1 Signaling Pathway Thai Khuc, Chia-Wen (Amy) Hsu, Srilatha Sakamuru, and Menghang Xia 4. Quantitative High-Throughput Luciferase Screening in Identifying CAR Modulators Caitlin Lynch, Jinghua Zhao, Hongbing Wang, and Menghang Xia 5. Transactivation and Coactivator Recruitment Assays for Measuring Farnesoid X Receptor Activity Chia-Wen (Amy) Hsu, Jinghua Zhao, and Menghang Xia 6. Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway Jinghua Zhao, Sunita J. Shukla, and Menghang Xia 7. Study Liver Cytochrome P450 3A4 Inhibition and Hepatotoxicity Using DMSO-Differentiated HuH-7 Cells Yitong Liu 8. Determination of Histone H2AX Phosphorylation in DT40 Cells Kana Nishihara, Sampada A. Shahane, and Menghang Xia 9. High-Throughput and High Content Micronucleus Assay in CHO-K1 Cells Sampada A. Shahane, Kana Nishihara, and Menghang Xia Part II: In Vivo Toxicological High-Thoughput Screening Methods 10. Better, Faster, Cheaper: Getting the Most Out of High-Throughput Screening with Zebrafish Lisa Truong, Michael T. Simonich, and Robert L. Tanguay 11. Fast Functional Germline and Epigenetic Assays in the Nematode Caenorhabditis elegans Zachary Lundby, Jessica Camacho, and Patrick Allard Part III: In Silico High-Thoughput Screening Toxcity Data Analysis 12. A Quantitative High-Throughput Screening Data Analysis Pipeline for Activity Profiling Ruili Huang 13. Correction of Microplate Data from High-Throughput Screening Yuhong Wang and Ruili Huang 14. CurveP Method for Rendering High-Throughput Screening Dose-Response Data into Digital Fingerprints Alexander Sedykh 15. Accounting Artifacts in High-Throughput Toxicity Assays Jui-Hua Hsieh 16. Accessing the High-Throughput Screening Data Landscape Daniel P. Russo and Hao Zhu 17. Curating and Preparing High Throughput Screening Data for Quantitative Structure Activity Relationship Modeling Marlene T. Kim, Wenyi Wang, Alexander Sedykh, and Hao Zhu